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Depth of Field refers to the depth of the specimen layer which is in sharp focus at the same time, even if the distance between the objective lens and the specimen plane is changed when observing and shooting the specimen plane by microscope. As human eyes are individually different in the ability of their focus adjustment, each person's perception of the focal depth varies.At present, the Berek formula is generally used, because it gives a focal depth value that often coincides with that obtained through experiments.

Depth of Field Formula

Visual Observation (Berek Formula)
Visual Observation (Berek Formula)

Video Camera

In the case of a video camera, the focal depth will vary according to number of pixels of CCD, optical magnification, and numerical aperture. The above-mentioned formula is used as a rough guide only.

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