This tutorial is one of many instructional videos
presented by the Olympus Industrial Equipment Group.
This tutorial video will provide you with detailed instructions
to perform count and measure with particle separtation
within the Olympus Stream Image-Analysis software.
Once you have your image captured
with the particles to be counted clearly defined
you will need to interface with the Count and Measure tool window
within the Processing layout.
First you will need to setup the threshold for your image.
Threshold is the software's ability to distinguish
between intensity values or color values
to mark them against a background.
Let's select the Automatic Threshold.
The Stream software will determine the best level
of threshold needed for the sample image.
This automatic detection works very well,
if for some reason it fails to capture all ponits of interest,
then you can hit cancel to change to manual threshold.
Clicking on the drop down will provide other methods
of thresholding within the software.
Manual Hue and Saturation Value (HSV threshold)
will provide ability to threshold on intensities
based on the HSV of the image.
Manual threshold will allow selection of individual color intensities
or grey value intensities,
Adaptive threshold will provide intensity selection
within grey scale on a color image.
Let's select Manual Threshold to demonstrate those capabilities.
With Manual Threshold open you now have the ability
to change the slider covering your histogram
to include specific levels of intensity for each color channel,
or by selecting to add or subtract ranges
with the dropper tools.
Let's cancel the manual threshold to use the automatic mode.
Again select the drop down to choose the Automatic Threshold mode.
Since automatic mode works very well on this colored image
to detect the threshold of each particle
we can now simply select Count and Measure
to perform the particle counting.
Further, there are options for count and measure
under the drop down next to button two.
Let's show these and go through them.
The first two options allow you to perform count and measure
on the entire image or a region of interest.
Below this you can define a new region of interest
by drawing a rectangle, circle, or polygon.
You can have multiple regions of interest to detect particles within.
This will provide you with phase measurements of particles
compared to area of the region of interest.
Detection Options allows you to change the settings
such as counting border particles and how to color code particles.
You can also choose options for your classifications from here,
or make adjustments to your current classifications.
Let's click out of this and continue with our inspection.
Now we can see the detected particles on our sample.
Since some particles were touching we will need to
perform particle separation to those ones.
You will need to click on the Select Detected Objects arrow
within the Count and Measure tool window.
This will allow you to click on specific particles
and highlight them to perform additional functions.
Now let's select a particle that is touching
and has been counted as one large particle.
We now need to decide to either manually split the particle
by drawing a border line between them by hand,
or to select the auto split option within the software.
Let's try the auto split option since this particle
can be easily determined as two particles based on it's shape.
The particle has now been split into two seperate particles
and the object measurement list was updated.
We can now deal with the other particles that are touching
and have been counted as one particle in a batch sequence.
First, select all the particles by clicking on the Object ID tab
within the Count and Meausure Results window.
That will highlight all particles detected.
Now select the Auto Split function again to run
on any particles that it can determine need to be separated.
Once it has performed any changes those will be shown
as new separated particles, and again the Object Measurement list was updated.
By double clicking on the Object Class field
we can sort the two classes generated
allowing us to easily show the different particles.
Let's click through the particles listed in class one.
This class has separated the particles by their shape factor.
This is the first class.
This is the second in the list.
As you can see this is a broken particle and we will need
to remove this particle entirely from our evaluation.
This is the third in the list.
And the final particle in class 1.
Now go back to the second particle listed
by clicking on it so we can remove it.
To remove or delete this particle you can simply
right click on the particle to bring up options.
Select Delete Selected Objects
to remove this partilce from the evaluation.
Once the particle has been removed
the Object Meausurement list will be updated.
Next let's show the object filter window.
Within this window you can perform
filtering of particles by any of our measurement methods.
Some examples would be to filter by size in area,
shape factor, diameter, or max length.
Filtering can provide a way to exclude
or include particles of interest.
You simple select the measurement
then select the minimum or maximum filter range
to allow you to manually select the minimum or maximum particles.
Or you can manually adjust the sliders
within the histogram to include or exclude certain ranges.
We will not be applying any filtering to this evaluation.
The next window will show the Class Measurements we set up.
This provides details on individual classes.
Class one is setup based on a Mean Shape Factor.
This allows the particles that are rectangular in shape
to be in class one and the round particles in class two.
We can show the Object Count for each class
along with any measurement value we would like.
The next window will show a histogram of the classes.
This histogram can be exported to a graph to be used
in the reporting tool as well by clicking on the export to chart button.
Let's go ahead and export this chart.
The Class Histogram is now opened as a chart.
This chart can be used in the reporting tool,
or saved into a database or file location.
Let's go back to the image by clicking on it at the top tab.
Now you will need to click back on the Object Measurement tab.
By selecting the export to workbook button,
the Object Measurement list will be transfered
to a usable workbook within the Stream.
Now that we have the list in a workbook
we can save it to the database or file location
or include it in our report.
In this instructional video, we have covered count and measure
with particle separation within the Olympus Stream Image-Analysis software.
We hope this video has been beneficial,
and we invite you to contact us directly with any questions.