This tutorial is one of many instructional videos
presented by Olympus Industrial Equipment.
This tutorial video will provide you with detailed instructions
to perform Multiple Image Alignment or MIA
by using manual stage control
within the Olympus Stream Image-Analysis software.
Manual MIA is available within Stream Essentials,
Motion, or Enterprise.
To begin you will need to have a live well focused image
on the screen and set the magnification you desire.
To create a more uniform stitched image
utilizing a manual exposure or locking the exposure
and shading correction are required.
You will interface with the "Process Manager" tool window.
Make sure to select Manual Processes.
Within the manual processes are instant EFI (extended focal imaging)
and manual MIA (multiple image alignment).
For this tutorial video we will select the manual MIA button.
Although Stream is capable of capturing "Instant EFI"
along with "Manual MIA."
Once the Manual MIA button is selected
you will notice the interface open.
This interface consists of direction arrows for motion of stitching.
To start the process you will need to select the Start Button
at the top of the tool window.
Once the process has started you will notice
the direction arrows will activate.
The arrows will define the stage scan path for the stitched image.
By clicking on one of the arrows
the software will move the captured image in the opposite direction
and generate a watermark and overlap the images
to allow you to manually move the stage
to align the next image frame.
The overlap amount between the two images
can be adjusted in the aquisition settings.
We will select the right arrow to start here.
Once the button was clicked, you will notice
the first captured image move to the left,
and a watermarked or transparent image is now displayed to the right,
you can now manually move the stage to overlap
the captured image with the new live image that is watermarked,
the alignment of the two images
needs to occur in the overlap area.
Once the overlap area is aligned for this first frame,
click on the down arrow to aquire the image below.
This will now generate the overlap and watermarked image
below the second captured frame.
You will have to manually adjust the stage
to align this next frame.
Next, click on the left arrow
to complete the square stitched area.
Proceed by adjusting the stage manually
to align the images within the overlap area.
Once you have completed this adjustment
you just have to click on the "Stop" button
within the process manager tool window.
Once the stop button has been pressed
the software will finalize the stitching of the last image,
and generate a new MIA image file.
You now have a fully stitched image that is calibrated
and ready to take measurements on or placed into a report.
In this instructional video, we have covered Manual MIA
within the Olympus Stream Image-Analysis software.
We hope this video has been beneficial,
and we invite you to contact us directly with any questions.