This tutorial is one of many instructional videos
presented by Olympus Industrial Equipment.
This tutorial video will provide you with detailed instructions
for generating automated MIA, or multiple image alignment,
within the Olympus Stream Image-Analysis software.
Automated MIA is available within Stream Motion or Enterprise.
To begin you will need to be within the "Acquisition" tab.
You should have a live image on the main screen.
The "Stage Navigator" tool window should also be open
through the "View" menu drop down, to generate the MIA.
To create a more uniform stitched image
utilizing a manual exposure or locking the exposure
and shading correction is required.
Within the "Stage Navigator" tool window you have two options
to set the region of area to be stitched for MIA.
The first is to use "Point and Click"
which allows you to click and drag a selection area
right on the overview image within the "Stage Navigator."
The second is to physically drive the stage
to two opposing points to define the area.
For this example choose "Point and Click."
After you select "Point and Click"
you will have to zoom into the overview image
by using the scroll wheel on the mouse.
This will allow you to mark the area of interest with more precision.
The overview image is a low magnification image
of the entire stage or sample.
Once you have zoomed into the area of interest
you simply point and click then drag the selection box
around the area to be stitched for the MIA.
Once the area is selected, the software will zoom back out
to show you the overall location of your selection.
If you use the scroll wheel on the mouse again,
you can zoom back into the area to determine
how many fields of view will be captured to generate the MIA.
Once you have confirmed the area is set,
you will need to select the "Process Manager" tool window.
Within the "Process Manager" tool window
you will want to make sure to select "Automatic Processes."
Within the automatic processes are EFI (extended focal imaging),
MIA (multiple image alignment),
Time Laps Imaging,
and the software Auto-focus function.
For this training select the MIA button, although Stream is capable
of capturing EFI and MIA images within the same scan.
Within the MIA Process window you have several buttons and options.
There is the ability to add stage locations to the overview image,
generate a new selection area by opposing stage positions,
ability to show the "Stage Navigator" tool window,
and turn on or off the focus map.
You can also select to overwrite the acquired positions
to clear old scans and capture all new.
The software can ignore any Z changes or focus changes
that may happen during the scan.
you can choose to use the focus map or
a hardware auto-focus if the system has this option.
To begin the process, simply select the "Start" button.
You will notice the "Process Manager" tool window
change once the scan has started.
The software will generate a new document
that starts blank and will fill in while the scan takes place.
At any point you can "Pause" or "Stop" the scan.
During the scan you can watch the progress
at the bottom left of the screen, or cancel it at any time.
Once the image is completed you will have a stitched image
that is calibrated and ready to take measurements on
and can be saved in any image format or inserted into a report.
In this instructional video, we have covered automated MIA
within the Olympus Stream Image-Analysis software.
We hope this video has been beneficial,
and we invite you to contact us directly with any questions.