This tutorial is one of many instructional videos
presented by the Olympus Industrial Equipment Group.
This tutorial video will provide you with detailed instructions
to capture EFI, extended focal images,
using manual focus within the Olympus Stream Image-Analysis software.
To begin you will need to have a live image within the "Acquisition" tab,
and interface with the "Process Manager" tool window.
You will need to select "Manual Processes."
The Manual Processes contain EFI (extended focal image)
and MIA (multiple image alignment).
The manual processes are available in Stream Essentials,
Motion or Enterprise.
You will want to select the Instant EFI button
to begin generating your extended focal image.
Once the settings open you have the ability to choose the Algorithm
to apply for they type of image you will be capturing.
The reflected light algorithm will capture the most in focus pixels
when using your light microscope or stereo microscope in brightfield mode.
Transmitted light (sqr) uses a quadratic weighting function
or Transmitted light (exp) uses an exponential function
to capture the focused pixels when using your light microscope
or stereo microscope in transmitted light mode.
The Stream software has the capability to correct for any frame shift
that will occur from a standard stereo microscope
due to off axis image capture.
Simply check mark the "Automatic frame shift"
to allow the software to align these frames during capture.
One you have the settings in place for
the type of image capture you will be performing
you simply select the "Start" button.
After hitting start, the main screen will change
to show three views of the image
The bottom left view is showing the building of the focus map,
the bottom right shows the live image at the current focal plane
you have it set to,
and the top main image is the EFI image
as it's being generated when you change focus.
Simply start adjusting the focus control on the microscope slowly
to allow the software to capture the most focus pixels
as you move from bottom to top of your sample's focus range.
There is no time limit to complete the sequence
and you can even go back through focus
if there was a spot you would like to improve.
Once you have scanned the focus from bottom level to top level
you will notice the focus map will be filled in
showing your EFI is complete,
you then need to click on the "Stop" button
in the "Process Manager" tool window.
The software will then display the completed EFI image
or all in focus image as a new document.
In this instructional video, we have covered manual EFI capture
within the Olympus Stream Image-Analysis software.
We hope this video has been beneficial,
and we invite you to contact us directly with any questions.