This tutorial is one of many instructional videos
presented by the Olympus Industrial Equipment Group.
This tutorial video will provide you with detailed instructions
for generating EFI, or extended focal images,
within the Olympus Stream Image-Analysis software.
To begin you will need to have a live image within the "Acquisition" tab,
and interface with the "Process Manager" tool window.
You will need to select "Automatic Processes."
The automatic processes contain EFI (extended focal image),
MIA (multiple image alignment),
Time Laps imaging, and the software Autofocus function.
The automatic processes are available in Stream Motion or Enterprise.
You will want to select the EFI button,
and for this demonstration only this one process will be selected,
although Stream can perform EFI and MIA processes within the same scan.
To start make sure to set focus at the bottom of your sample,
either by using the hardware focus knob
or the focus buttons within the software.
Starting at the bottom of focus
insures the image will not be inverted.
Once this bottom focus point has been reached
you will want to select the "Start" set point.
You will now need to drive focus to the top level of the focus range,
or the highest point of your sample.
This can be done using the up arrow buttons within the "Process Manager"
or by using the hardware control focus knob on the microscope frame.
Once this point has been reached, you must set the "End" point.
You must verify the "Recommended Step Size" value is set,
if the step size is too far above the recommended step size
your image will come out with blur spots.
You can hit the "Apply" button to have it default to the recommended value.
The software sets this value automatically
based on the function of the objective lens numerical aperature.
Once the step size has been determined it will display the number of Z slices
required for this magnificaiton and Z range.
You will need to make sure to check mark "Extended Focal Imaging"
at the bottom of the Process Manager window.
This will allow the software to automatically generate the EFI,
or all in focus image once it's done scanning the Z steps.
Once the settings are complete
you simply press the "Start" button at the top.
The Process will now be automated and perform all of the image captures
required to generate the all in focus image or EFI.
You can watch the progress of the automation at the bottom
of the image window, or cancel it at any time.
Once the process is complete the software will generate a new document.
This finalized image is now an all in focus or EFI image
and can be saved in all normal image formats.
You can now perform 3D measurements on the EFI image,
or render it in 3D with the optional "Surface View" module.
In this instructional video, we have covered generating EFI images
within the Olympus Stream Image- Analysis software.
We hope this video has been beneficial,
and we invite you to contact us directly with any questions.