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This tutorial is one of many
instructional videos

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presented by the Olympus Industrial
Equipment Group.

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This tutorial video will provide
you with detailed instructions

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to perform
layer thickness measurements

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on cross sectioned samples

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within the Olympus Stream
Image-Analysis software.

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The layer thickness
solution is an add-in module

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for Stream Essentials and above.

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To begin we need to
have a captured image

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of a cross sectioned sample with
open layers

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in the OLYMPUS Stream software.

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For measurement and evaluation
of the image

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we will want to be within
the "Processing" tab.

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Within the "Materials Solutions"
tool window

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we will choose "Layer Thickness"
to start that specific solution.

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Once the "Layer Thickness"
window opens

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it will start with the "Image source"
selection,

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the option to load settings,

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and the ability to skip the
sample information section.

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At the top of the window
we have "Instructions"

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that will guide us through
each screen,

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and at the bottom we have the
work flow list of this solution.

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The image source selection
can be from a new live image,

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the selected image already opened
within the software,

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a batch of images located
within a system folder,

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or a batch of images you select

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within the optional
OLYMPUS Stream database.

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The ability to process
multiple images as a batch

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will increase workflow
and productivity

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also allowing you to average data
over a group of images

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associated with a single sample.

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Opening the settings at the
start of this process

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will allow the utilization
of standard values

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to increase repeatability
in measurement results,

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and also decrease the process time.

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Click on the next arrow
icon at the bottom

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to move to the
"Sample Information" section.

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Within "Sample Information"
we have the ability

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to provide titles or names
to the part or sample,

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provide a title for the
group of images,

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give detailed comments for
that group of images,

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and also provide detailed comments
for each individual image

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being processed for the sample.

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Click the next arrow to
move onto settings.

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Within 'Settings' we have the ability
to choose

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how the contours are
to be defined.

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The choices are Automatic definition,

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Magic Wand definition,

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or Manual definition.

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Automatic definition is set to
define the contours

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based on a difference in
threshold value

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between the background and layer.

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The software will automatically
determine this difference.

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Magic Wand still utilizes a
threshold evaluation

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of the layer but will allow you
to manually adjust

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the amount of threshold
that is applied

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to where the selection is made.

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Manual definition allows you
to manually place the points

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to define the contours
for the selected layer.

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This is useful when you do
not have enough threshold

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or contrast difference between
the layer and the background.

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Select 'Automatic' for
this demonstration

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as the sample image does
have a contrast difference

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between the layer and the background.

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We also have the choice
of layer type at the bottom.

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With an open layer type,
you can measure layer structures

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that continue through the image.

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When you have a closed layer type,

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you can measure
circular layer structures.

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In closed layer,
the contour line's first point

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is automatically connected
to its last point.

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Once you have the settings completed

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you can click on the next arrow
to move to 'Automatic'.

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At the top of this window,
several options

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with which we can specify details

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concerning the background
can be found.

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Select the Dark option
if the image displays

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a dark layer in front
of a bright background.

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Select the Bright option
if the image displays

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a bright layer
in front of a dark background.

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Or we can let the software
automatically determine the setting.

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For this image select 'Automatic'

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to allow the software to
set the background selection.

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After setting the background
selection click on the next arrow

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to move to 'Define Borders'.

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We can now define our borders

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based on the background
contour selections

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we defined in the previous window.

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Click on 'Define borders'.

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Now simply select the
first contour line

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you wish to define by clicking on it.

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After we select that line,
the software will then allow us

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to place the marker points at
each end of the contour line.

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We will place the first
point to the left side

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then the second point
to the right side

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marking our entire contour line.

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Since this line stands alone
we need to right click

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to finish marking our points.

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It will then turn the
select contour line blue.

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Now we can select the next
contour line to define our layer.

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Select the line at the
bottom of the layer

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which we defined the first contour.

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Once again we will mark
this contour line

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with a beginning and end point.

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Since this contour line was connected

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with the definition of the dark layer,

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we can also mark the
contour line below

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with two points using the
blue cross marks.

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Once we have completed the
definition of the borders

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we right click to finish.

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Click the next arrow
to 'Edit borders'.

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Within 'Edit borders'
we can add more contours,

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move borders, delete borders,
connect borders,

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split borders, and smooth borders.

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These functions allow you to provide

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touch ups needed before
taking your final measurements.

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Let's try the function
for smoothing a border in this sample.

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Select 'Smooth borders' button,

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then simply click on the
border we want to smooth out,

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and the desired amount
will be applied.

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This function simply allows
us to straighten out

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some of the effects of a
jagged edge of a layer border

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to assist in placement
of the measurement points.

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After we select the border
right click to finish editing.

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Click the next arrow to
go on to 'Define layers'.

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Within 'Define layers'
we need to add the borders

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to define our individual layers.

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Click on 'Add layers' button to begin.

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Next we need to click on the
top border to our first layer,

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and then click on the bottom border.

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The software will automatically
place a fiber line

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or midpoint in green
for the selected layer.

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Next we click on the top
border for the second layer,

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which is the same border for
the bottom of the first layer,

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and then click on the bottom
border for the second layer.

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Right click to finish defining layers.

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Now that we have both layers defined
we will see them in the list.

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Click on the next arrow to
continue on to our 'Image results'.

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We now can see the individual
measurement results of the two layers.

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We can select layer 2 here to display

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the yellow measurement lines
for the second layer.

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We can also display all
measurement lines

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at once by selecting
the show all button.

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To select a different measurement type

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click on the down arrow button.

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There are three types of measurements
we can use for the layer.

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Parallel, Perpendicular,
and Shortest Distance.

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The parallel is our default one

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and aligns the measurements parallel
to each other along the layer.

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Perpendicular will align them
perpendicular

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with the neutral fiber line in green.

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And shortest distance will only
show the measurement lines

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with the shortest distance
in the layer.

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If we were to use the
closed layer option

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we would have another selection here
for displaying the measurement lines

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to lie in a radially
symmetrical pattern.

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If we leave the 'Display all
measurements' selected here

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it will carry over into our report.

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Click next to move onto 'Results'.

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Within 'Results' we will see the
mean thickness value

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for all of our selected
layers for this sample,

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and we have the ability to select

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to generate a report and a workbook.

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We will select both here and
then click the next arrow.

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Since we selected 'to
generate a report,'

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this will now take us to
the 'Reporting' window

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and allows us to select
either the default template,

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or a user defined template.

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We can also define the
content of the report below.

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We will leave all of the default
values selected here.

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Click the 'Finish' button to
complete the report

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and generate the workbook.

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Once the report generates
it will open within Microsoft Word.

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The first page shows
the 'Analysis summary'.

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The second page shows
the 'Sample results'.

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The third page will display the image
and the individual layer measurements.

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The report can now be saved as a
word document locally

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or into the optional database.

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Going back to the Stream software

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we can also view the workbook
that was generated.

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The first tab displays the 'Summary'.

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The second tab displays
the 'Sample' results.

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The third tab displays the individual
line measurements for both layers,

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and the measurement statistics
at the bottom.

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This workbook can be saved locally
or within the database,

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and can also be exported
to Excel directly.

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In this instructional video, we have
covered layer thickness measurement

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within the Olympus Stream
Image-Analysis software.

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We hope this video
has been beneficial,

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and we invite you to contact us
directly with any questions.