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Proper microscope setup is critical

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to help ensure optimal
performance and safety

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when making observations
or using a microscope camera.

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Proper microscope setup
increases operator productivity

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and efficiency
for a streamlined workflow.

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For stereomicroscopes,
three of the most important

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and often overlooked elements
of microscope setup are:

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Interpupilary adjustment

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Diopter and zoom
parfocality adjustment

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Camera and microscope parfocality

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Adjusting the interpupillary
distance is necessary

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to obtain proper binocular vision.

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First, place a flat sample
on the stage.

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Adjust the lighting and bring
the sample into focus.

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While holding the left and right
observation tubes with both hands,

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look through the eyepieces.

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Adjust for binocular vision
by moving the eyepieces

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together or apart until the
left and right fields of view

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merge to form a single image.

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For operators who wear glasses,

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Olympus recommends
using the eye shades

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in the normal fold-down position

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to help prevent eyeglasses
from being scratched.

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Next, the stereomicroscope
must be properly focused

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to provide a clear image
from low to high magnification.

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The diopter and zoom parfocality

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must be properly adjusted to provide

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consistently clear images through
the range of magnifications.

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The diopter adjustment is used

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to compensate for differences
between one’s eyes

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and is an important step
in preventing eye strain

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that can lead to fatigue as well as
eliminating the tedious task

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of having to re-focus the microscope
every time the zoom is changed.

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To make the adjustment,
turn the diopter rings

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of the left and right eyepieces
to position “0”.

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For operators with perfect
20/20 vision,

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this is the correct setting.

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All others require
the following adjustments.

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First, place a simple, large sample

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that will be easy to observe
on the stage plate.

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Set the zoom adjustment knob
for the lowest magnification

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and rotate the focus adjustment knob

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to bring the sample into focus.

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Next, set the zoom adjustment knob
for the highest magnification

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and rotate the focus adjustment knob
to bring the sample into focus.

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Finally, set the zoom adjustment knob
for the lowest magnification

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and rotate the diopter adjustment ring
of the left and right eyepieces

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to bring the sample into focus.

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The adjustment is now complete

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and the image should now
remain in focus

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from low to high zoom magnification.

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For stereomicroscopes that are
used by multiple operators,

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Olympus recommends that each
operator record their diopter settings

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since they will vary between users.

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For stereomicroscopes equipped
with digital imaging systems,

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proper camera parfocality is important

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to help ensure that the image
viewed through the microscope

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and the image displayed on the monitor

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are in focus with each other.

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For this procedure to work,

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the microscope should be
equipped with a camera mount

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that has a built-in focus adjustment.

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First, place a sample
on the stage plate.

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While looking through the eyepieces,

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bring the image into focus
using the microscope focus knob.

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Display the image on the monitor.

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If the image on the
monitor screen is blurry,

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a parfocality adjustment is required.

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To adjust, simply turn the focus
dial on the camera adapter

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until the image on the monitor
screen is in focus.

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If your camera adaptor
has a locking mechanism,

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lock it into place.

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By making the three simple
microscope adjustments

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discussed in this video,

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users will be able to
make more comfortable

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and efficient observations.

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For more information on Olympus
Industrial Microscope solutions

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contact your local representative

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or visit our website at
www.olympus-ims.com.